LOCUS       40924_48353        3891 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZE1PLlacO-1 luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Lutz R, Bujard H.
  TITLE     Independent and tight regulation of transcriptional units in 
            Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 
            regulatory elements
  JOURNAL   Nucleic Acids Res. 25 (6), 1203-1210 (1997)
  PUBMED    9092630
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Lutz R, Bujard H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-1996) Zentrum fuer Molekulare Biologie, University
            of Heidelberg, Im Neuenheimer Feld 282, Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 3891)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1997"; volume: "25"; issue: "6"; pages: 
            "1203-1210"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-AUG-1996) Zentrum fuer Molekulare Biologie, University of 
            Heidelberg, Im Neuenheimer Feld 282, Heidelberg 69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    9..25
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    32..48
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             90..101
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             108..1757
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      1776..1862
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2026..2614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2702..2796)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2829..3686)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3687..3791)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac
       61 atcagcagga cgcactgacc gaattcatta aagaggagaa aggtaccatg gaagacgcca
      121 aaaacataaa gaaaggcccg gcgccattct atccgctaga ggatggaacc gctggagagc
      181 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
      241 cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag
      301 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
      361 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
      421 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg
      481 tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa
      541 taatccagaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
      601 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt
      661 cctttgatcg tgacaaaaca attgcactga taatgaactc ctctggatct actgggttac
      721 ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc
      781 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
      841 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
      901 tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg
      961 cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg
     1021 atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg
     1081 aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga
     1141 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     1201 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     1261 atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc
     1321 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     1381 actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca
     1441 aaggatacca ggtggccccc gctgaattgg agtcgatatt gttacaacac cccaacatct
     1501 tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     1561 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     1621 aagtaacaac cgccaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     1681 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     1741 gcggaaagtc caaattgtaa tgactctaga ggcatcaaat aaaacgaaag gctcagtcga
     1801 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     1861 atccgccgcc ctagacctag gcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2221 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga ctagtgcttg
     2701 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     2761 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgta aacttggtct
     2821 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2881 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2941 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3001 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3061 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3121 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3181 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3241 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3301 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3361 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3421 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3481 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3541 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3601 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3661 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3721 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3781 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     3841 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca c
//
